Technical Data
N3006-14
Nod2, CT (Caspase Recruitment Domain 15, CARD15)
Description:
Description:
Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2 (2). NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF- B, which is regulated by its carboxy-terminal
leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn’s disease (3,4) that is a main type of chronic inflammatory bowel disease.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide, 14aa at the carboxy terminus of human NOD2 (GenBank accession no. Q9HC29).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Recognizes NOD2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Inohara N, Koseki T, del Peso L, et al. Nod1, an Apaf-1- like activator of caspase-9 and nuclear factor-kappaB. J. Biol. Chem. 1999; 274:14560-7.
2. Ogura Y, Inohara N, Benito A, et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB. J. Biol. Chem. 2001; 276:4812-8.
3. Hugot JP, Chamaillard M, et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 2001; 411:599-603.
4. Ogura Y, Bonen DK, Inohara N, et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 2001; 411:603-6. (RD1105)