Technical Data
Nod2, CT (Caspase Recruitment Domain 15, CARD15)
Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2 (2). NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF- B, which is regulated by its carboxy-terminal
leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohns disease (3,4) that is a main type of chronic inflammatory bowel disease.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceRabbit
As reported
Synthetic peptide, 14aa at the carboxy terminus of human NOD2 (GenBank accession no. Q9HC29).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes NOD2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Inohara N, Koseki T, del Peso L, et al. Nod1, an Apaf-1- like activator of caspase-9 and nuclear factor-kappaB. J. Biol. Chem. 1999; 274:14560-7.
2. Ogura Y, Inohara N, Benito A, et al. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB. J. Biol. Chem. 2001; 276:4812-8.
3. Hugot JP, Chamaillard M, et al. Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease. Nature 2001; 411:599-603.
4. Ogura Y, Bonen DK, Inohara N, et al. A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease. Nature 2001; 411:603-6. (RD1105)