Technical Data
Nod2 (Nucleotide Binding Oligomerization Domain 2, NOD2 Protein, NOD2B, Arthrocutaneouveal Granulomatosis, ACUG, BLAU, Caspase Recruitment Domain 15, CARD15, CD, Inflammatory Bowel Disease Protein 1, IBD1, LRR Containing Protein, PSORAS1)
NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry, and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100-1:2000. Tested on NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates. Endogenous detection has some background. Immunofluorescence/ Immunocytochemistry: 1:10-1:500
Immunohistochemistry (Paraffin): 1:10-1:500
Immunoprecipitation: 1:10-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG19A46Affinity Purified
100ul-20CBlue IceHumanMouse
Recombinant corresponding to aa28-301 of human NOD2. Species Sequence Homology: primate, 92%.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human Nod2 at ~110kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lala, S., et al. Crohn's disease and the NOD2 gene: a role for the paneth cells. Gastroenterology. 125(1) 47-57 (2003) 2. Ogura, Y., et al. Expression of NOD2 in paneth cells: a possible link to Crohn's ileitis. Gut. 52 1591-1597(2005)