		Technical Data
N3006-14B	Nod2 (Nucleotide Binding Oligomerization Domain 2, NOD2 Protein, NOD2B, Arthrocutaneouveal Granulomatosis, ACUG, BLAU, Caspase Recruitment Domain 15, CARD15, CD, Inflammatory Bowel Disease Protein 1, IBD1, LRR Containing Protein, PSORAS1)

Description:
NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: Tested on NOD2 transfected 293T lysates and HT29 human colorectal adenocarcinoma lysates. Endogenous detection has some background.
Immunohistochemistry: Paraffin
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	9A46	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~2mg/ml

Immunogen:

Recombinant corresponding to aa28-301 of human NOD2. Cellular Localization: Cytoplasmic.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in Tris-glycine buffer, 150mM sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes human Nod2 at ~110kD. Species Sequence Homology: primate, 92%.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lala, S., et al. Crohn's disease and the NOD2 gene: a role for the paneth cells. Gastroenterology. 125(1) 47-57 (2003) 2. Ogura, Y., et al. Expression of NOD2 in paneth cells: a possible link to Crohn's ileitis. Gut. 52 1591-1597(2005)