Technical Data
N3006-14D
Nod2 (Nucleotide Binding Oligomerization Domain 2, NOD2 Protein, NOD2B, Arthrocutaneouveal Granulomatosis, ACUG, BLAU, Caspase Recruitment Domain 15, CARD15, CD, Inflammatory Bowel Disease Protein 1, IBD1, LRR Containing Protein, PSORAS1)
Description:
NOD2 is a member of the apoptosis regulating protein family that includes caspase recruitment-domains, as well as Apaf-1 and NOD1. It contains two N-terminal CARDs, a nucleotide binding domain (NBD), and multiple C-terminal leucine-rich repeats (LRRs). NOD2 is expressed in monocytes (whereas NOD1 is expressed in multiple tissues). NOD2 plays a role in regulating NF-kappaB, acts as an intracellular receptor for bacterial lipopolysaccharides, and contributes to inflammatory bowel disease (IBD) and Crohn's disease.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A synthetic peptide made to the C-terminal region of the human NOD2 protein sequence (between residues 1,000-1,040).
Purity:
Purified by immunoaffinity chromatography.
Form
Tris-glycine buffer containing 150mM NaCl.
Specificity:
Specific for NOD2 protein. Species Crossreactivity: This antibody reacts with human protein. Other species have not been tested. The immunogen used for this antibody production has 100% homology with human and monkey protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hisamatsu, T., et al. CARD15/NOD2 funstions as an antibacterial factor in human intestinal epithelial cells. Gastroenterology. 124(4):1145-1149, 2003. (Immunoprecipitation, Western Blot, Human)
2. Abbott. D.W., et al. New signaling step for key player in chron's disease. Cell Press. 2004
3. Abbott, D.W., et al. The Crohn's disease protein, NOD2, requires RIP2 in order to induce ubiquitinylation of a novel site of NEMO. Current Biology. 14(24): 2217-2227, 2004.
4. Costans, A. Giving a Nod2 the right target. The Scientist. 22-26, 2005.
5. Barnich, N., et al. GRIM-19 interactswith nucleatide oligomerization domain 2 and serves as downstream effector of anti-bacterial function in intestinal epithelial cells. J. of Biol. Chem. 280(19):19021-19026, 2005.
6. Berrebi, D., et al. Card 15 gene overexpression in mononuclear and epithelial cells of the inflamed Crohn's disease colon. Gut. 52:840-846, 2003. (Human, Immunohistochemistry, Immunofluoresence)
7. Chamaillard, M., et al. Gene-environment interaction modulated by allelic heterogeneity in inflammatory diseases. PNAS. 100(6): 3455-3460, 2003. (Human, Western Blot)