Technical Data
N5200
Neutrophils, Apoptotic
Description:
Anti-Neutrophils, Apoptotic binds to the surface of apoptotic neutrophils. Neutrophils have been implicated in the pathogenenesis of a variety of inflammatory diseases including the adult respiratory distress syndrome, idiopathic pulmonary fibrosis, ulcerative colitis and rheumatoid arthritis. Although the neutrophil is a vital component of the body’s defense against infectious agents, uncontrolled release of its toxic substances may inflict “friendly fire” damage on surrounding tissue and propagate the inflammatory response, leading to scarring and tissue destruction. Apoptosis leads to recognition and safe disposal of dying cells by phagocytosis. Binding of the monoclonal antibody to apoptotic neutrophils is dependent on the presence of sialoglycoprotein fetuin, a constituent of bovine serum. Fetuin is the antigen for this monoclonal antibody, and together form a complex in solution that is necessary and sufficient for binding to apoptotic neutrophils. The antigen recognized may act as a “molecular bridge” between the surface of the apoptotic neutrophil and the phagocyte to modulate apoptotic neutrophil clearance at inflammatory sites. Human macrophage phagocytosis of apoptotic neutrophils was augmented in vitro by addition of the antigen recognized, which does not bind to other apoptotic leukocytes.

Applications:
Suitable for use in Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10
Flow Cytometry: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19G243Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
Human myeolmonocytic cell line THP-1
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity:
Recognizes human apoptotic neutrophils.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hart, S et al; Specific binding of an antigen-antibody complex to apoptotic human neutrophils. Am J Pathol 2003, 162: 1011