Technical Data
N5201
Neutrophils, Apoptotic
Description:
Neutrophils are recruited in large numbers to inflammation sites. Clearance of these cells from the inflammatory site is done by programmed cell death (apoptosis) of the neutrophil. As a consequence, many cellular mechanisms are down-regulated in the apoptotic neutrophil such as: adhesion, chemotaxis, phagocytosis, enzyme secretion, generation of a respiratory burst, and responsiveness to external stimuli. Changes in the cell surface of dying neutrophils are recognized by macrophages, which destroy the neutrophils by phagocytosis. Many changes in the amount of proteins, carbohydrates and lipids on the surface of neutrophils are associated with the apoptotic event. Apoptotic Neutrophils (BOB78) is an up-regulated protein, molecular weight 90 kDa, that appears in the granular compartment of the late apoptotic neutrophil. This antigen does not appear in freshly isolated erythrocytes, platelets, lymphocytes, monocytes, or neutrophils. Monoclonal antibodies specific for BOB78 are a useful tool for studying the biology of apoptotic neutrophils.

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
THP-1 cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM,k10H1Purified
SizeStorageShippingSourceHost
100ug4C/-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human whole apoptotic neutrophils BOB78.
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1M Tris-Glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Apoptotic Neutrophils.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.