Technical Data
N5202
Neutrophils, Apoptotic
Description:
Neutrophils have been implicated in the pathogenenesis of a variety of inflammatory diseases including the adult respiratory distress syndrome, idiopathic pulmonary fibrosis, ulcerative colitis and rheumatoid arthritis. Although the neutrophil is a vital component of the body’s defense against infectious agents, uncontrolled release of its toxic substances may inflict “friendly fire” damage on surrounding tissue and propagate the inflammatory response, leading to scarring and tissue destruction. Apoptosis leads to recognition and safe disposal of dying cells by phagocytosis. Binding of the monoclonal antibody BOB93 to apoptotic neutrophils is dependent on the presence of sialoglycoprotein fetuin, a constituent of bovine serum. Fetuin is the antigen for BOB93, and BOB93 and fetuin form a complex in solution that is necessary and sufficient for binding to apoptotic neutrophils. The antigen recognised by BOB93 may act as a “molecular bridge” between the surface of the apoptotic neutrophil and the phagocyte to modulate apoptotic neutrophil clearance at inflammatory sites. Human macrophage phagocytosis of apoptotic neutrophils was augmented in vitro by addition of the antigen recognised by BOB93, which does not bind to other apoptotic leukocytes.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human PBMC's.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10H2Affinity Purified
SizeStorageShippingSourceHost
100ug4°C/-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human whole apoptotic neutrophils BOB93.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1M Tris-Glycine, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes human Apoptotic Neutrophils.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.