Technical Data
N5349-08
NOS (uNOS, Universal Nitric Oxide Synthase)
Description:
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen sections): 1:100
Western Blot: 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgMNOS-3F7-B11-B5Ascites
SizeStorageShippingSourceHost
200ul-20CBlue IceBovineMouse
Concentration:
Not determined
Immunogen:
Purified neuronal nitric oxide synthase (nNOS; NOS I) from bovine brain.
Purity:
Ascites
Form
Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
Specificity:
Recognizes iNOS (NOS II), eNOS (NOS III) and nNOS (NOS I) in mouse, rat and bovine samples. Detects bands of ~130kDa (representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS)), ~155kDa (in tissues expressing nNOS) and ~140kDa (in tissues expressing eNOS) by Western blot.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia: C. Xue, et al.; Am. J. Physiol. 267, L667 (1994) Abstract