Technical Data
N5349-08B
NOS (uNOS, Universal Nitric Oxide Synthase)
Description:
Nitric Oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells including vasorelaxation, neurotransmission and cytotoxicity. NO is produced by a Nitric Oxide Synthase (NOS) family of enzymes which oxidizes the amino acid L-arginine to form L-citrulline and NO.Two constitutive forms, brain NOS (bNOS) & endothelial cell NOS (eNOS), and one inducible form (iNOS), have been cloned showing regions for binding of calmodulin, NADPH, FAD, and FMN. iNOS has been found in a variety of tissues including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. It is induced after exposure to cytokines such as interferon-* (IFN), tumor necrosis factor, interleukin-1, interleukin-2, and lipopolysaccarides (LPS). After cytokine induction, iNOS has a delayed response followed by high levels of NO produced over a long period of time. eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells. eNOS is capable of synthesizing NO in vascular endothelial cells where it appears to play an important role in the control of vasotension and platelet aggregation.

Applications:
Suitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM9A76Ascites
SizeStorageShippingSourceHost
200ul-20CBlue IceBovineMouse
Concentration:
Not determined
Immunogen:
Purified bovine bNOS.
Purity:
Ascites
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Detects brain Nitric Oxide Synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS). Species Crossreactivity: Rat, Mouse and bovine. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Xue C et al. Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia. Am J Physiol 267:L667-78 (1994). Rengasamy A et al. Immunohistochemical demonstration of a paracrine role of nitric oxide in bronchial function. Am J Physiol 267:L704-11 (1994). Nathan C Nitric oxide as a secretory product of mammalian cells. FASEB J 6:3051-64 (1992).