		Technical Data
N5349-08B	NOS (uNOS, Universal Nitric Oxide Synthase)

Description:
Nitric Oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells including vasorelaxation, neurotransmission and cytotoxicity. NO is produced by a Nitric Oxide Synthase (NOS) family of enzymes which oxidizes the amino acid L-arginine to form L-citrulline and NO.Two constitutive forms, brain NOS (bNOS) & endothelial cell NOS (eNOS), and one inducible form (iNOS), have been cloned showing regions for binding of calmodulin, NADPH, FAD, and FMN. iNOS has been found in a variety of tissues including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. It is induced after exposure to cytokines such as interferon-* (IFN), tumor necrosis factor, interleukin-1, interleukin-2, and lipopolysaccarides (LPS). After cytokine induction, iNOS has a delayed response followed by high levels of NO produced over a long period of time. eNOS and bNOS share ~50% sequence homology, and their activity depends on binding to the calcium/calmodulin complex. Both constitutive isoforms respond immediately to increased levels of calcium to produce low levels of NO over a short period of time. bNOS has been found in neurons, peripheral nerve cells, macula densa & pancreatic islet cells. eNOS is capable of synthesizing NO in vascular endothelial cells where it appears to play an important role in the control of vasotension and platelet aggregation.

Applications:
Suitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgM	9A76	Ascites

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Bovine	Mouse

Concentration:

As reported

Immunogen:

Purified bovine bNOS.

Purity:

Ascites

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

Specificity:

Detects brain Nitric Oxide Synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS). Species Crossreactivity: Rat, Mouse and bovine. Not yet tested in other species.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

This product has been used in:Xue C et al. Distribution of NOS in normoxic vs. hypoxic rat lung: upregulation of NOS by chronic hypoxia. Am J Physiol 267:L667-78 (1994). Rengasamy A et al. Immunohistochemical demonstration of a paracrine role of nitric oxide in bronchial function. Am J Physiol 267:L704-11 (1994). Nathan C Nitric oxide as a secretory product of mammalian cells. FASEB J 6:3051-64 (1992).