Technical Data
Notch3 (Notch homolog 3, Drosophila, hN3, CASIL, CADASIL)
The Notch protein sits like a trigger penetrating the cell membrane, with part of it inside and part outside. Proteins when binding to the exterior part cause the release of a portion of the interior part, which typically then makes its way to the cell nucleus to alter gene expression. The Notch gene belongs to a family of epidermal growth factor (EGF)-like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1,Notch2, Notch3 and Notch4(Int3), which have been implicated in the differentiation of the nervous system and other structures. The EG- like proteins Delta and Serrate have been identified as ligands of Notch1.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 1:100-1:300
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Synthetic peptide corresponding to the C-terminal region of the human NOTCH homolog 3 (NOTCH3) protein.
Purified by affinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human Notch3. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bellavia D, et al: Combined expression of pTalpha and Notch3 in T cell leukemia identifies the requirement of preTCR for leukemogenesis. Proc. Natl. Acad. Sci. U S A. 99: 3788-3793 (2002). 2. Ito D, Tanahashi N, Murata M, Sato H, Saito I, Watanabe K, Fukuuchi Y: Notch3 gene polymorphism and ischaemic cerebrovascular disease. J. Neurol. Neurosurg. Psychiatry. 72: 382-384 (2002). 3. Sugaya K, Fukagawa T, Matsumoto K, Mita K, Takahashi E, Ando A, Inoko H, Ikemura T: Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3. Genomics 23: 408-419 (1994).