Technical Data
N5375-26D
Notch3, CT (Notch Homolog 3 (Drosophila), hN3, Neurogenic Locus Notch Homolog Protein 3, CADASIL, CASIL)
Description:
NOTCH3 is the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human NOTCH3 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human NOTCH3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Suwanwela, N., et al., J Med Assoc Thai 86(2):178-182 (2003). 2.Ahearn, E.P., et al., Am. J. Med. Genet. 114(6):652-658 (2002). 3.Bellavia, D., et al., Proc. Natl. Acad. Sci. USA 99(6):3788-3793 (2002). 4.Ito, D., et al., J. Neurol. Neurosurg. Psychiatr. 72(3):382-384 (2002). 5.Joutel, A., et al., Lancet 350(9090):1511-1515 (1997).