Technical Data
N5375-27C
Notch3 (Neurogenic Locus Notch Homolog Protein 3)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 1x10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from Armenian hamsters.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. DyLight conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG12G38Affinity Purified
SizeStorageShippingSourceHost
100ug4C/-20CBlue IceMouseHamster
Concentration:
~1mg/ml
Immunogen:
Mouse Notch 3-Fc fusion protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes mouse Notch-3. Species Crossreactivity: Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moriyama, Y. et al. (2008) Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice. Int. Immunol. 20: 763-773. 2. Sekine, C. et al. (2009) Differential regulation of splenic CD8- dendritic cells and marginal zone B cells by Notch ligands. Int. Immunol. 21: 295-301.