Technical Data
N5375-27F
Notch3 (Neurogenic Locus Notch Homolog Protein 3, Notch 3, Notch-3) (PerCP)
Description:
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early event developmental processes. The extracellular domains of Notch receptors interact with the extracellular domains of the transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. Notch-3 has the same biochemical mechanism of signal transduction as Notch-1, where a series of cleavage events release the Notch intracellular domain (NICD). NICD translocates to the nucleus and initiates transcription of Notch responsive genes. Thus, Notch acts as both a ligand binding receptor and a nuclear factor that regulates transcription. Human Notch-3 shows 90% aa identity to mouse Notch-3 over the entire protein.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Absorption: 482nm and 564nm (maximum)

Emission: 675nm (maximum)

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG112K144Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do Not FreezeBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa40-467 from human Notch-3 expressed in Sf21 cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with PerCP.
Specificity:
Recognizes human Notch-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363. 2. Mizutani, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9026.