Technical Data
N5380-08
NR1, phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor)
Description:
NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1ug/ml detects NR1 on 10ug of lambda phosphatase untreated and treated rat microsomal brain tissue lysates.
Immunohistochemistry: 1:25 detects NR1 in normal rat hippocampus tissue.
Optimal dilutions to be determined by the researcher.

Positive Control:
Lambda phosphatase untreated and treated rat microsomal brain tissue lysates

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to rat NR1 phosphorylated at Ser896 (KLH). Epitope: Phosphorylated Ser896
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150 mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes rat NR1 when phosphorylated at Ser896. Species sequence homology: human, mouse 100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.