Technical Data
N5380-08
NR1, phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor)
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Technical Data |
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N5380-08 |
NR1, phosphorylated (Ser896) (NMDA Receptor, N-methyl-D-aspartate Receptor) |
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Description: NR1 (NMDA1or NMDAR1, also known as GRIN1) along with the NR2 subunit forms a heterodimer that make up the NMDA receptor. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml detects NR1 on 10ug of lambda phosphatase untreated and treated rat microsomal brain tissue lysates. Immunohistochemistry: 1:25 detects NR1 in normal rat hippocampus tissue. Optimal dilutions to be determined by the researcher. Positive Control: Lambda phosphatase untreated and treated rat microsomal brain tissue lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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