Technical Data
N5380-25B
NR2A (Grin2a, Glutamate Receptor, ionotropic, NMDA2A (epsilon 1), GluRepsilon1, NMDAR2A, N-methyl D-aspartate Receptor subtype 2A)
Description:
NR2 or glutamate receptor, ionotropic, N-methyl D-aspartate 2 (NMDA2; NMDAR2) along with the NR1 subunit forms a heterodimer that make up the NMDA receptor. By alternative splicing there are various isoforms of NR2 classified as NR2A through D. The NMDA receptor (NMDAR) is characterized as a ionotropic receptor for glutamate. As the name suggests, (NMDA (N-methyl D-aspartate) is an agonist for the receptor. A distinct characteristic of the NMDA receptor is that it is both ligand-gated and voltage-dependent based on its sensitivity to magnesium. The NR1 subunit binds glycine while the NR2 subunit binds glutamine for activation. Activated NMDA receptors result in the opening of an ion channel that is nonselective to cations. The result is the flow of Na+ and small amounts of Ca2+ ions into the cell and K+ out of the cell. Calcium flux by the NMDARs has been implicated in synaptic plasticity, a mechanism in the cell for learning and memory.

Applications:
Suitable for use in Western Blot. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:3000 detects NR2A on 10ug of rat brain microsomal tissue lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat brain microsomal tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11C250Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
~0.1mg/ml
Immunogen:
Histidine-tagged recombinant protein corresponding to the C-terminus of mouse NR2A. Species sequence homology: human and mouse.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 40% glyceol.
Specificity:
Recognizes NR2A. Species crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.