Technical Data
N5385-01E
NRAS, ID (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, AV095280, GTPase NRas, HRAS1, N-Ras Protein Part 4, NRAS1, OTTHUMP00000013879, OTTMUSP00000023521, Transforming Protein N-Ras, v-Ras Neuroblastoma RAS Viral Oncogene Homolog)
Description:
NRAS is a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. This protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in the gene encoding this protein are a cause of juvenile myelomonocytic leukemia (JMML).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 1:32,000 & 0.01-0.03ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-RMKKLNSSDDGTQ from an internal region of human NRAS.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human NRAS. Species sequence homology: Canine, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.