Technical Data
N5399-05
NT-1 (Neurotrophin 1, B Cell Stimulating Factor 3, BCSF-3)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Indirtect and Sandwich ELISA: > 0.5ug/ml using 100ul/well antibody solution detects 0.2-0.4ng/well of recombinant hNNT-1/BCSF-3.
Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O, and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Highly pure (> 98%) recombinant human NT-1 (novel)/BCSF-3.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 40-50% glycerol, sterile dH2O to 0.1-1mg/ml.
Specificity:
Recognizes human NT-1 (novel)/BCSF-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.