Technical Data
N6000-07E
NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)
Description:
NT-3 is a neurotrophic factor structurally related to NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (238 total amino acid residues).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry: 0.5-2ug/ml
Optimal dilutions to be determined by the researcher.

Reconstitution: Desired volume with sterile distilled water.

Suggested concentration: 0.51mg/mL.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
20ug4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.5-1mg/ml
Immunogen:
An 11 amino acid peptide corresponding to the amino-terminal of mouse Neurotrophin 3 (NT3) coupled to BSA.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS. No preservatives added.
Specificity:
Recognizes Neurotrophin 3 (NT3). Does not crossreact with BDNF or NGF. Species Reactivity: Human, rat, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Brain Res. 621:189199 (1993). Brain Res. 643:162172 (1994). J. Comparative Neurology 363:6977 (1995). J of Neuroscience 16:25082521 (1996)