Technical Data
N6000-07E
NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2)
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Technical Data |
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N6000-07E |
NT-3 (Neurotrophin 3, HDNF, NGF-2, Nerve growth factor-2) |
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Description: NT-3 is a neurotrophic factor structurally related to NGF, BDNF, and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. NT-3 is expressed by neurons of the central nervous systems and can signal through the trk receptors. NT-3 promotes the growth and survival of nerve and glial cells. The amino acid sequences of human, murine and rat NT-3 are identical. Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kD polypeptide monomers (238 total amino acid residues). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml Immunohistochemistry: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Reconstitution: Desired volume with sterile distilled water. Suggested concentration: 0.5–1mg/mL. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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