		Technical Data
N6000-26C	NTAL (Non-T Cell Activation Linker, HSPC046, Linker for Activation of B cells, LAB, Lat2, Membrane Associated Adapter Molecule, Williams Beuren Syndrome Chromosome Region 5, WBSCR5, WSCR5, WBSCR15)

Description:
Alternatively termed as linker for activation of B cells (LAB), NTAL is expressed in plasma membrane lipid rafts of B cells, mast cells, monocytes and NK cells. The engagement and activation of antigen receptors on B and T lymphocytes initiates signal transducing pathways that are vital for the successful initiation of an adaptive immune response. It is following the cross-linking of the B cell receptor (BCR) or high-affinity FE receptors of myeloid cells, that the tyrosine phosphorylation of NTAL occurs, enabling the formation of complexes between NTAL and further signaling molecules Grb2, Gab1, Sos1 and c-Cbl. Recent studies into the functions of NTAL, have indicated that this molecule may also act as a negative regulator of mast cell Fc Epsilon RI-mediated signalling.

Applications:
Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry (Frozen). Other applications not tested.

Recommended Dilution:
Western Blot: Non-reducing, detects a band of approximately 30kD in Ramos cell lysates.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6A454	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant cytoplasmic domain (aa 91-244) of human NTAL.

Purity:

Purified by protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

Specificity:

Recognizes non T-cell activation linker (NTAL), a 30kD transmembrane adaptor protein that is structurally related to the T cell adaptor protein LAT. Species Cross Reactivity:Human, Mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Brdicka, T. et al. (2002). Non-T cell Activation Linker (NTAL): A transmembrane adaptor protein involved in immunoreceptor signaling. J. Exp. Med. 196(12):1617-1626. 2. Wange, R.L. (2003). The missing Link(er): A return to symmetry in antigen receptor signaling? Molecular Interventions 3(2):75-78. 3. Koonpaew, S. et al. (2004). The importance of three membrane-distal tyrosines in the adaptor protein NTAL/LAB. J. Biol. Chem. 279(12):11229-11235.