Technical Data
NTAL (Non-T Cell Activation Linker, HSPC046, Linker for Activation of B cells, LAB, Lat2, Membrane Associated Adapter Molecule, Williams Beuren Syndrome Chromosome Region 5, WBSCR5, WSCR5, WBSCR15)
Alternatively termed as linker for activation of B cells (LAB), NTAL is expressed in plasma membrane lipid rafts of B cells, mast cells, monocytes and NK cells. The engagement and activation of antigen receptors on B and T lymphocytes initiates signal transducing pathways that are vital for the successful initiation of an adaptive immune response. It is following the cross-linking of the B cell receptor (BCR) or high-affinity FE receptors of myeloid cells, that the tyrosine phosphorylation of NTAL occurs, enabling the formation of complexes between NTAL and further signaling molecules Grb2, Gab1, Sos1 and c-Cbl. Recent studies into the functions of NTAL, have indicated that this molecule may also act as a negative regulator of mast cell Fc Epsilon RI-mediated signalling.

Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry (Frozen). Other applications not tested.

Recommended Dilution:
Western Blot: Non-reducing, detects a band of approximately 30kD in Ramos cell lysates.
Optimal dilution determined by the researcher.

Recommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:HRP

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG16A454Affinity Purified
100ug-20CBlue IceHumanMouse
Recombinant cytoplasmic domain (aa 91-244) of human NTAL.
Purified by protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Recognizes non T-cell activation linker (NTAL), a 30kD transmembrane adaptor protein that is structurally related to the T cell adaptor protein LAT. Species Cross Reactivity:Human, Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brdicka, T. et al. (2002). Non-T cell Activation Linker (NTAL): A transmembrane adaptor protein involved in immunoreceptor signaling. J. Exp. Med. 196(12):1617-1626. 2. Wange, R.L. (2003). The missing Link(er): A return to symmetry in antigen receptor signaling? Molecular Interventions 3(2):75-78. 3. Koonpaew, S. et al. (2004). The importance of three membrane-distal tyrosines in the adaptor protein NTAL/LAB. J. Biol. Chem. 279(12):11229-11235.