Technical Data
N6000-27
NTH (Endonuclease III Like 1, NTHL1, OCTS3)
Description:
Oxygen radicals damage chromosonal DNA causing cell death and inducing mutations. Although a major focus of oxidatively damaged DNA has centered on the repair of 8-oxo-G, a number of other damaged DNA sites are created by free-radical attack on DNA. The human NTH1 repair protein is one enzyme that has been shown to act on a large number of these other oxidatively damaged DNA sites (2).

A homologue of E. coli Endonuclease III, NTH is a DNA glycosylase with apurinic/apyrimidinic lyase activity. The NTH protein has broad substrate specificity, including numerous ring saturation and fragmentation products of pyrimidines. Research indicates NTH plays a crucial role in removal of oxidative base lesions in mitochondrial DNA (3).

Application(s):
Suitable for use in Western Blot where a band at ~37kD is seen on SDS-PAGE. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1,000-1:5,000 (ECL)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Full-length recombinant protein (human)
Purity:
Serum
Form
Supplied as a liquid in 0.1% sodium azide.
Specificity:
Specific for human NTH protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.