|Cloning||Storage: -70°CShipping: Dry Ice|
Purified from Alteromonas espejiana BAL31 culture medium. It is a mixture of two enzyme species designated as the slow (S) and fast (F) forms because of their different kinetic properties. Nuclease BAL31 catalyses the degradation of single-stranded and linear duplex DNA or RNA to 5'-mononucleotides. The linear duplex DNA or RNA is degraded progressively from both 5'-and 3'-ends without the introduction of internal scissions. The enzyme also cleaves at nicks, gaps, single-stranded regions or other lesions of duplex DNA or RNA. It requires Ca2+ and Mg2+ for both exo-and endonucleolytic activities.
1 unit of enzyme removes 600 base pairs from each end of 2ug of linearised pBR322 DNA in 10min at 30°C in 20ul of the reaction mixture containing 20mM Tris-HCl (pH 8.1), 600mM NaCl, 12.5mM CaCl2, 12.5mM MgCl2, 0.1mM EDTA.
2X Reaction Buffer:
40mM Tris-HCl (pH 8.1 at 25°C), 25mM CaCl2, 25mM MgCl2, 1.2MNaCl
20mM Tris-HCl (pH 8.1), 100mM NaCl, 5mM CaCl2, 5mM MgCl2, 1mM EDTA and 50% glycerol.
Tested for the absence of endodeoxyribonucleases.
Removing of nucleotides from the termini of double-stranded DNA DNA restriction mapping
Detection of lesions or distorted structures in duplex DNA
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