Technical Data
N6900
Nuclease BAL31
50u
Cloning Storage: -70°CShipping: Dry Ice
Purified from Alteromonas espejiana BAL31 culture medium. It is a mixture of two enzyme species designated as the “slow” (S) and “fast” (F) forms because of their different kinetic properties. Nuclease BAL31 catalyses the degradation of single-stranded and linear duplex DNA or RNA to 5'-mononucleotides. The linear duplex DNA or RNA is degraded progressively from both 5'-and 3'-ends without the introduction of internal scissions. The enzyme also cleaves at nicks, gaps, single-stranded regions or other lesions of duplex DNA or RNA. It requires Ca2+ and Mg2+ for both exo-and endonucleolytic activities.
 Concentration:
1–5u/ul.

Unit Definition:
1 unit of enzyme removes 600 base pairs from each end of 2ug of linearised pBR322 DNA in 10min at 30°C in 20ul of the reaction mixture containing 20mM Tris-HCl (pH 8.1), 600mM NaCl, 12.5mM CaCl2, 12.5mM MgCl2, 0.1mM EDTA.

2X Reaction Buffer:
40mM Tris-HCl (pH 8.1 at 25°C), 25mM CaCl2, 25mM MgCl2, 1.2MNaCl

Storage Buffer:
20mM Tris-HCl (pH 8.1), 100mM NaCl, 5mM CaCl2, 5mM MgCl2, 1mM EDTA and 50% glycerol.

Quality Control:
Tested for the absence of endodeoxyribonucleases.

Applications:
• Removing of nucleotides from the termini of double-stranded DNA • DNA restriction mapping
• Detection of lesions or distorted structures in duplex DNA
1. Wei Ch.-F. et al., J. Biol. Chem. 258:13506–13512 (1983)
2. Gray, H.B. et al., Nucleic Acids Res. 2:1459–1492 (1975
3. Bencen, G.H. et al., J. Biol. Chem. 259:3584 (1984)
4. Legerski, R.J. et al., Nucleic Acids Res. 5:1445–1463 (1978)
5. Talmadge, K., Gilbert, W., Gene 12:235–241 (1980)
6. Ponz, M. et al., Proc. Natl. Acad. Sci USA, 79:4298–4302 (1982)
7. Hauser, C.R., Gray, H.B.Jr., Gene. Anal. Tech. Appl. 8:139–147 (1991)
8. Zhen, W.-P. et al., Biochemistry 25:6598–6603 (1986)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.