Technical Data
N7100-70B
5'-Nucleotidase, Recombinant, Human (CD73, 5'-nucleotidase, ecto)
10ug
Molecular Biology Storage: -20°CShipping: Blue Ice
CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside-5'-monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. These functions include the adhesion of B-cells to follicular dendritic cells and T-cell signaling CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'-Nucleotidase activity. The native enzyme is a homodimer bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor. This recombinant human CD73 is truncated at the C-terminus, and lacks the GPI modification site. The recombinant CD73 is therefore secreted as a monomer.

Recombinant protein corresponding to Trp27-Lys547, with a C-terminal 6-His tag from human 5'-Nucleotidase expressed in CHO cells (AAH65937).

SDS-PAGE:
61-62kD under reducing conditions

Biological Activity:
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate. The specific activity is 15,000pmoles/min/ug.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
59kD
Source: CHO cells
Purity: 90% by SDS­PAGE under reducing conditions and visulized by silver stain. Endotoxin: 1EU/ug (LAL)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris, pH 7.5, 120mM sodium chloride, 4mM calcium chloride, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Resta, R. et al. (1998) Immunol. Rev. 161:95. 2. Pilcher, M. et al. (2003) J. Biol. Chem. 278:13468. 3. Airas, L. (1998) Leuk. Lymphoma 29:37. 4. Resta, R. and Thompson. L.F. (1997) Cell Signal. 9:131. 5. Jalkanen, S. and Salmi, M. (2008) Arterioscler. Thromb. Vasc. Biol . 28:18.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.