		Technical Data
N7100-71B	5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

Description:
CD73 (Ecto-5'nucleotidase, 5 '-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.

Applications:
Suitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml .Detection limit for rmCD73 is
~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. Detection limit for rmCD73 is ~1ng/lane under non-reducing and reducing conditions.
Flow Cytometry (Murine splenocytes): 25ug/ml. Add 100ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not excedding 200ul.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20ºC. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Mouse	Sheep

Concentration:

~0.2mg/ml

Immunogen:

CHO cell-derived rmCD73. (aa 29-549)

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.

Specificity:

Recognizes mouse CD73.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.