Technical Data
N7100-71C
5'-nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT, E5NT, eN, eNT, NT, NT5, NTE)
Description:
5'-nucleotidase (CD73) is a 70kD GPI-anchored cell surface molecule and belongs to the 5’-nucleosidase family. CD73 is useful as marker for lymphocyte differentiation. It is abundantly expressed on the vascular endothelium and at a low level on certain subpopulations of human lymphocytes. Like many glycosyl-phosphatidylinositol (GPI)-anchored molecules, it transmits potent activation signals in T cells when ligated by antibodies. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Ecto-5’-nucleotidase activity is an important mediator of the anti-inflammatory effect by converting extracellular AMP into a potent anti-inflammatory substance adenosine. CD73 has been shown to function as a co-stimulatory molecule in human T cells and to have a role in regulating lymphocyte adhesion. Triggering of CD73 on the surface of lymphocytes, but not on endothelial cells, results in the shedding of the CD73 and increased adhesion of lymphocytes to endothelium via LFA-1 clustering. Furthermore, CD73 has been implicated to mediate homing of skin-infiltrating lymphocytes in vivo. In B-cell chronic lymphocytic leukemia the expression of CD73 is decreased. Besides this, CD73 activity has been implicated as sensitive and useful indicator for mild zinc deficiency.
Applications:
Suitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Western Blot and Functional Studies. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: HUVEC cells were seeded on gelatin-coated coverslips and stained with N7100-71C (5).
Flow Cytometry: 1:50. Stains the extracellular domain (1).
Immunohistochemistry (Frozen): 1:50. Tissue sections were fixed in acetone. Not suitable for use with paraffin sections (5).
Functional Studies: Functions as an inhibitor of lymphocyte binding to HUVEC. Tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer (1).
Optimal dilutions to be determined by the researcher.
Postitive Control: HUVEC and fresh lymphocytes
Negative Control: COS cells
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG19G246Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Inflamed synovial stroma from rheumatoid arthitis patients.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes membrane bound and soluble human 5'-nucleotidase. Causes a reduction in 5'-nucleotidase expression on lymphocytes, reduces enzyme activity, and inhibits the binding of lymphocytes to endothelial cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Niemelä, J et al; IFN-alpha induced adenosine production on the endothelium: a mechanism mediated by CD73 (ecto-5’-nucleotidase) up-regulation. J Immunol 2004, 172: 1646 2. Airas, L et al; CD73 engagement promotes lymphocyte binding to endothelial cells via a lymphocyte function-associated antigen-1-dependent mechanism. J Immunol 2000, 165: 5411 3. Airas, L et al; Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells. J Cell Biol 1997, 136: 421 4. Airas, L et al; CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73. J Exp Med 1995, 182: 1603 5. Airas, L et al; Lymphocyte-vascular adherion protein-2 is a novel 70-kDa molecule involved in lymphocyte adhesion to vascular endothelium. J Immunol 1993, 151: 4228