Technical Data
N7100-71F
5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase) (PE)
|
Technical Data |
|
N7100-71F |
5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase) (PE) |
|
|
Description: CD73 (also known as [ecto]-5’-nucleotidase/5’-NT) is a variably glycosylated, 69-73kD member of the 5’-nucleotidase family of enzymes. It is expressed on multiple cell types, including vascular endothelium, transitional and nonkeratinized epithelium, cardiomyocytes, small intestine epithelium, FoxP3 Treg lymphocytes, FDCs, and B cells. CD73 hydrolyzes AMP to adenosine and phosphate. This creates diffusible nucleosides necessary for cell homeostasis, and a ligand for cell membrane adenosine receptors. Mature human CD73 is a 523aa GPI-linked protein (aa27-549). It contains a large Zn-dependent nucleotidase catalytic region (aa28-532) and a C-terminal substrate binding site (aa500-506). On the cell surface, it exists as a disulfide-linked homodimer. Two splice variants are reported. One shows a deletion of aa405-454, and a second possesses a 12aa substitution for aa253-574. Over aa1-511, human CD73 shares 88% aa identity with both mouse and rat CD73. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, CHO-derived, recombinant human 5’ Nucleotidase/CD73. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||