		Technical Data
N7100-71M	5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

Description:
CD73 is a 70kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T- and B-cells; mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 co-stimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000
Immunohistochemistry (Formalin fixed paraffin embedded): 1:200-1:400
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	1D7	Ascites

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Recombinant corresponding to a fragment of human NT5E.

Purity:

Ascites

Form

Supplied as a liquid, 0.03% sodium azide.

Specificity:

Recognizes human NT5E.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.