Technical Data
N7100-72
5'-nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT, Nt5e, Nt5, Nte)
Description:
CD73 (ecto-5`-nucleotidase) is a 69kD GPI-anchored surface protein.In mouse, expression of CD73 in bone marrow is restricted to the CD11b+ myeloid cells.In spleen it is largely expressed on T cells.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.06ug/10e6 cells in 100ul volume
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kAffinity Purified
SizeStorageShippingSourceHost
25ug-20°CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
CHO cells transfected with mouse CD73
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes mouse CD73.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yamashita et al 1998. Eur. J. Immunol. 28:2981. 2. Resta RY et al. 1998. Immunol. Rev. 161:95.