		Technical Data
N8341-05	NuMA (Nuclear Mitotic Apparatus Protein, NuMA Protein, NUMA1, SP-H Antigen)

Description:
Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
Western Blot: With mitotic cells, detects a main band at ~210kD and additional weaker bands at 240 and180kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgM	A73-B/D12	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Live Ls 174T cells (colon carcinoma)

Purity:

Purified

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes human NuMA. Reacts specifically with a cell nucleus, mitotic cell antigen, poles of the mitotic spindle, adjacent microtubuli and centrosomes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Butschak, G. et al., New monoclonal antibodies recognizing phosphorylated proteins in mitotic cells. Acta Histochem. 97: 19-31 (1995). 2. Compton, D.A. et al., Primary structure of NuMA, an intranuclear protein that defines a novel pathway for segregation of proteins at mitosis. J.Cell Biol. 116: 1395-1408 (1992).