Technical Data
Media
Nutrient Gelatin (Powder)
N8535
Microbiological Grade
Storage RT    Shipping RT
Formulation* per Liter:
Pancreatic Digest of Gelatin5g
Beef Extract3g
Gelatin120g
Final pH: 7.0 0.2 at 25.0C
* Grams per liter may be adjusted or formula supplemented to obtain desired performance
Nutrient Gelatin is used to determine gelatin liquefaction by proteolytic microorganisms. Gelatin was the first gelling agent used to solidify liquid culture media, thus bacterial counts could be determined and isolation of pure cultures obtained. However, when using gelatin incubation temperature of 20C was required and it was observed that many organisms were capable of liquefying the gelatin. Agar eventually replaced gelatin as a solidifying agent. Nutrient gelatin is recommended for the taxonomic differentiation of the Enterobacteriaceae and nonfermenting gram-negative bacteria. The rate of liquefaction is used as a differential characteristic.

This medium is primarily useful when investigating the liquefaction characteristics of pure cultures of organisms having minimal to no fastidious nutritional needs.

Quality Control Specifications:
1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is clear to opalescent and light to medium yellow.
3. Expected cultural response after 1 to 7 days at 35 2C.

Organism Growth Gelatinase
Bacillus subtilis ATCC 9372 Growth +
Clostridium perfringens ATCC 13124 Growth +
Escherichia coli ATCC 25922 Growth -
Staphylococcus aureus ATCC 25923 Growth +

Storage: Store the sealed bottle containing the dehydrated medium at 2-30C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.


Mix 128 grams of the medium in a Liter of purified water until evenly dispersed. Heat at 50C with repeated stirring to dissolve medium entirely. Distribute and autoclave at 121C for 15 minutes.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.