		Technical Data
N8574-10	NYREN18, NT (NEDD8 Ultimate Buster-1, NUB1, Negative Regulator Of Ubiquitin-like Proteins 1, Renal Carcinoma Antigen NY-REN-18, NUB1)

Description:
NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the N-terminal region of human NYREN18 (KLH).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human NYREN18. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tanaka, T., et al., J. Biol. Chem. 278(35):32905-32913 (2003). 2. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 3. Kito, K., et al., J. Biol. Chem. 276(23):20603-20609 (2001). 4. Kamitani, T., et al., J. Biol. Chem. 276(49):46655-46660 (2001). 5. Scanlan, M.J., et al., Int. J. Cancer 83(4):456-464 (1999).