Technical Data
N8574-10-AP
NYREN18, NT (NEDD8 Ultimate Buster-1, NUB1, Negative Regulator Of Ubiquitin-like Proteins 1, Renal Carcinoma Antigen NY-REN-18, NUB1) (AP)
Description:
NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human NYREN18 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Labeled with Alkaline Phosphatase.
Specificity:
Recognizes human NYREN18. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tanaka, T., et al., J. Biol. Chem. 278(35):32905-32913 (2003). 2. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 3. Kito, K., et al., J. Biol. Chem. 276(23):20603-20609 (2001). 4. Kamitani, T., et al., J. Biol. Chem. 276(49):46655-46660 (2001). 5. Scanlan, M.J., et al., Int. J. Cancer 83(4):456-464 (1999).