Technical Data
O3000-04
OATP1 (Organic Anion Transporting Polypeptide 1)
Description:
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; OATP1-3, LST-1 and PGT), have been cloned and characterized from various tissues. The OATP family of proteins share significant sequence homology and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N- and C-terminus).
Rat OATP, now called OATP1, encodes a protein of 670aa. OATP1 was localized at the apical portions of the S3 segment of the proximal tubule and at the apical surface of the choroid plexus of rat brain. It is also found in liver, lung, skeletal muscle and colon. It mediates Na+-independent uptake of wide range of amphipathic substrates including sulfobromopthalein (BSP), bile acids, estrogen conjugates and neutral steroids, etc. Human liver OATP (670aa) is only 67% homologous with rat OATP1. It differs in functional properties from rat OATP1 and is highly expressed in brain.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Western Blot: 1-10ug/ml using ECL.
Immunohistochemistry: 1-20ug/ml (FFPE).
Optimal dilutions to be determined by the researcher.

Control Peptide:
O3000-05: OATP1, Control Peptide, Rat

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 17aa near the mid region of rat OATP1, conjugated to KLH. Species Sequence Homology: mouse- 47%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes rat OATP1. No significant sequence homology is detected with other OATP2-3 or human OATP or LST-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Higuchi, K., Hepatology Res. 29: 60-66 (2004). 2. Martin, G.G., Biochem. J. 391: 549-560 (2005). 3. Tanaka, Y., Biochem. Biphys. Res. Commun. 309: 324-330 (2003). 4. Kuroda, M., J. Gastroenterology Hepatology 19(2): 146-153 (2004). 5. Jacquemin, E., et al., PNAS 91: 133-137 (1994). 6. Kullak-Ublick, G.A., et al., Gastroenterol. 109: 1274-1282 (1995).