Technical Data
O3001-08
OATP2 (Organic Anion Transporting Polypeptide 2)
Description:
According to recent nomenclature this hepatocyte-specific protein is identical to OATP1B1 (2) also known as OATP-C.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: Boiling sample before SDS-PAGE is not recommended. Samples should be kept at 37ºC for 30 min.
Immunohistochemistry (Frozen): Neat; Suggested incubation time: 1 hr at RT.
Optimal dilutions to be determined by the researcher.

Positive Control:
SCL21A6 transfected HEK293 cells

Negative Control:
HepG2 and PLC cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM5F253Supernatant
SizeStorageShippingSourceHost
5ml-20°CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the C-terminus (21aa) of human organic anion transporter SLC21A6 (KLH).
Purity:
Supernatant
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human OATP2 at ~90kD. Using immunofluorescence microscopy this antibody stains the sinusoidal (basolateral) membranes in frozen sections of human liver, hepatocellular carcinoma and the human cell line HEK293 transfected with SLC21A6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cui, Y., et al., Lab. Invest. 83: 527-538 (2003). 2. Hagenbuch, E. & Meier, P.J., Biochim. Biophys. Acta 1609: 1-18 (2003).