Technical Data
O3001-09
OATP2 (Organic Anion Transporting Polypeptide 2)
Description:
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs and other organic anions from the blood by a process called “renal secretion.” Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway and placenta. Several multispecific OATs (OAT1-3, OAT-K1, OATK2) and OATPs (oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). OAT2, previously called NLT (novel liver-specific transporter) shows 42% homology with OAT2. OAT2 (rat 535 aa; human 548 aa) is predominantly expressed in liver, and to a lower level in kidney. OAT2 mediates sodium-independent, multispecific organic anion transport (PAH, salicylate and acetylsalicylate, prostaglandin E2, and dicarboxylate).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml
Western Blot: 1-10ug/ml using ECL.
Immunohistochemistry (paraformaldehyde fixed): 2-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
A synthertic peptide corresponding to 15aa near the N-terminus, extracellular domain of rat OATP2 (035913), conjugated to KLH. Species Sequence Homology: mouse; 85%, human; 78%.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes rat OATP2 (Organic Anion Transporting Polypeptide 2). No significant sequence homology is detected with other OATs or other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Naud, J. et al., (2008) Drug Metabolism & Disposition 36:124-128. 1. Wong, H.; Toxicol. Sci. 84: 232-242 (2005). 2. Freeman, W.M.; Neuroscience 108: 371-380 (2001). 3. Kuroda, M.; J. Gastroenterol. Hepatol. 19: 146-153 (2004). 4. Higuchi, K.; Hepatology Research 29: 60-66 (2004). 5. Noe, B., et al; PNAS 94: 10346-10350 (1997). 6. Abe, T., et al; J. Biol. Chem. 273, 22395-22401 (1998). 7. kakyo, M., et al; FEBS lett. 445, 343-346 (1999).