Technical Data
O3003-20
Organic Anion Transporter 3 (OAT3)
Description:
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Recently OAT3 (rat 536-aa, human 568 aa) has been cloned that is most closely related to OAT1 (~49% identity). Rat OAT3 is expressed in the liver, brain, kidney and eye. OAT3 also mediates Na-independent uptake of several organic anions (PAH, ochratoxin, and estrone sulfate, etc). OAT3 shows 92% homology with mouse Roct (reduced in osteosclerosis transporter), possibly an ortholog of rat OAT3. As compared to OAT1-2, strong expression of OAT3 in brain suggests its role in removing organic anion in the brain.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Coat with ELISA plates with Control Peptide at 1ug/ml.
Immunohistochemistry: 2-20ug/ml
Immunofluorescence: 2-20ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide: O3003-22

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 16aa near the N-terminus, cytoplasmic domain of rat OAT3 (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in PBS, 0.1% BSA.
Specificity:
Recognizes rat OAT3. No significant sequence homology is detected with other OATs or other proteins. Species sequence homology: mouse, 93%; human and rabbit, 87%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kwak Jin oh, 2005, IUBMB Life, 57 , p109-117, WB, IF/IP 2. Bahn A, 2005, Am J Physiol Cell Physiol, 289: C1075 - C1084, IF 3. Lash LH, 2006, Toxicology, 228, 200-218, WB 4. Hawkins TB, 2007, Neuroscience Letters, 411, 1-5, WB, IF 5. Schneider R, 2007, Am J Physiol Renal Physiol, 292: F1599 -F1605 6. Lash LH, 2007, Chemico-Biological Interactions, 170, 124-134, WB 7. Kushuhara, H., et al. (1999) J. Biol. Chem. 274, 13675-13680. 8. Race, J.E., et al. (1999) BBRC 255, 508-514.