Technical Data
O3003-20
Organic Anion Transporter 3 (OAT3)
Description:
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Recently OAT3 (rat 536aa, human 568aa) has been cloned that is most closely related to OAT1 (~49% identity). Rat OAT3 is expressed in the liver, brain, kidney and eye. OAT3 also mediates Na-independent uptake of several organic anions (PAH, ochratoxin, and estrone sulfate, etc). OAT3 shows 92% homology with mouse Roct (reduced in osteosclerosis transporter), possibly an ortholog of rat OAT3. As compared to OAT1-2, strong expression of OAT3 in brain suggests its role in removing organic anion in the brain.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Coat with ELISA plates with Control Peptide at 1ug/ml.
Immunohistochemistry: 2-20ug/ml
Immunofluorescence: 2-20ug/ml
Optimal dilutions to be determined by the researcher.

Control Peptide: O3003-22

Storage and Stability:
May be stored at 4░C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20░C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20░CBlue IceRatRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 16aa near the N-terminus, cytoplasmic domain of rat OAT3, conjugated to KLH (NP_112622.1). Species sequence homology: mouse, 93%; human and rabbit, 87%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as liquid in PBS, 0.1% BSA.
Specificity:
Recognizes rat OAT3. No significant sequence homology is detected with other OATs or other proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kwak Jin oh, 2005, IUBMB Life, 57 , p109-117, WB, IF/IP 2. Bahn A, 2005, Am J Physiol Cell Physiol, 289: C1075 - C1084, IF 3. Lash LH, 2006, Toxicology, 228, 200-218, WB 4. Hawkins TB, 2007, Neuroscience Letters, 411, 1-5, WB, IF 5. Schneider R, 2007, Am J Physiol Renal Physiol, 292: F1599 -F1605 6. Lash LH, 2007, Chemico-Biological Interactions, 170, 124-134, WB 7. Kushuhara, H., et al. (1999) J. Biol. Chem. 274, 13675-13680. 8.áRace, J.E., et al. (1999) BBRC 255, 508-514.