Technical Data
OATP (Organic anion transporting polypeptide)
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
O3003: OATP, Human, Control Peptide (Organic anion transporting polypeptide)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
A 15aa peptide internal sequence (middle regions) of human OATP (KLH). Epitope: ~C-terminus extracellular domain (UniProt # P46721). Species sequence homology: chimp 100%.
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Recognizes human OATP. No significant sequence homology is detected with other oatp1-3 or human LST-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jacquemin E et al (1994) PNAS 91: 133-137; Kullak-Ublick, GA et al (1995)2. Gastroenterol. 109:1274-1282 3. Gao, Exp. Eye Res. 80: 61-72 (2004).