Technical Data
O3003-29
OATP (Organic anion transporting polypeptide)
Description:
Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3, LST-1, and PGT), have been cloned and characterized from various tissues. OATPs family of proteins share significant sequence homology, and general secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
O3003: OATP, Human, Control Peptide (Organic anion transporting polypeptide)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
A 15aa peptide internal sequence (middle regions) of human OATP (KLH). Epitope: ~C-terminus extracellular domain (gene accession # P46721). Species sequence homology: chimp 100%.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% BSA.
Specificity:
Recognizes human OATP. No significant sequence homology is detected with other oatp1-3 or human LST-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jacquemin E et al (1994) PNAS 91: 133-137; Kullak-Ublick, GA et al (1995)2. Gastroenterol. 109:1274-1282 3. Gao, Exp. Eye Res. 80: 61-72 (2004).