|Biochemicals||Storage: -20°CShipping: Blue Ice|
Okadaic Acid, a marine toxin, polyether derivative produced by dinoflagellates, is a potent, cell-permeable, specific inhibitor of PP-1 and PP-2 serine/threonine-speci c protein phosphatases in a variety of cell types.
Storage and Stability:
Light Sensitive. Store at -20°C. Stable as a powder for at least 12 months. Stable as a liquid for 6 months.
Warning: This product is a tumor promoter. Read the Material Safety Data Sheet (MSDS) prior to rehydration and use. Initial rehydration of the product should be performed under a chemical fume hood using extreme caution.
Source: Prorocentrum concavum
Purity: 98% (HPLC)
Form: Supplied as a lyophilized powder. Soluble in DMSO, 100% ethanol or 100% methanol at 1mg/ml. Use only fresh solutions.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Haystead et al., Nature 337:78, 1989
Redpath and Proud, Biochem. J. 262:69, 1989
Takai, J. Mus. Res. & Cell Motil. 9:563, 1988 Wheat et al., Mol. Cell. Biol. 14:5881, 1994
Amara, F. M. et al.: Defining a Novel cis-Element in the 3’-Untranslated Region of Mammalian Ribonucleotide Reductase Component R2 mRNA. J. Biol. Chem. (1996) 271, 20126-20131: Cell culture.
Mimnaugh, E. G. et al.: Possible Role for Serine/Threonine Phosphorylation in the Regulation of the Heteroprotein Complex between the hsp90 Stress Protein and the pp60v-src Tyrosine Kinase. J. Biol. Chem. (1995) 270, 28654-28659: Cell culture.
Wheat, W.H. et al.: Simian virus 40 small tumor antigen inhibits dephosphorylation of protein kinase A-phosphorylated CREB and regulates CREB transcriptional stimulation. Mol. Cell. Biol. (1994) 14, 5881-5890: Inhibitor in protein phosphatase assay.
Trichostatin A (19-138)
El-Osta, A., et. al. Precipitous Release of Methyl-CpG Binding Protein 2 and Histone Deacetylase 1 from the Methylated Human Multidrug Resistance Gene (MDR1) on Activation. Molecular and Cellular Biology (2002) 22: 1844-1857. Chromatin Immunoprecipitation
Calyculin A (19-139)
Gautreau, A., et.al. Morphogenic Effects of Erzin Require a Phosphorylation Induced Transition from Oligomers to Monomers at the Plasma Membrane. The Journal of Cell Biology (2000) 150: 193-204. Serine/Threonine Inhibition
LY 294002 (19-142)
Wei, Q., et. al. Pitx2a Expression Alters Actin-Myosin Cytoskeleton and Migration of HeLa Cells through Rho GTPase Signaling. Mol. Biol. Cell (2002) 13: 683-697.PI3K Inhibition
PD 98059 (19-143)
Wei, Q., et. al. Pitx2a Expression Alters Actin-Myosin Cytoskeleton and Migration of HeLa Cells through Rho GTPase Signaling. Mol. Biol. Cell (2002) 13: 683-697. MEK1 Inhibition