Technical Data
Oncostatin M, Recombinant, Human (OSM)
Molecular Biology Storage: -20CShipping: RT
Recombinant corresponding to 227aa human Oncostatin M expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50 <2ng/ml ( > 5x105units/mg). The biological activity is determined by measuring the oncostatin M concentration dependent stimulation of proliferation of TF-1 cells. The suggested concentration range for in vitro activity studies is 0.2-20.0ng/ml. The optimal concentration should be determined for each specific application. Oncostatin M is observed to exert variable, cell type dependent effects. For example, oncostatin M is observed to stimulate the growth of normal fibroblasts and AIDS-related Kaposi sarcoma cells, but is observed to inhibit the growth of A375 melanoma cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: ~98% (SDS-PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. No preservative added. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.