Technical Data
O6500-02A
Oncostatin M, Recombinant, Human, aa26-234, His-Tag (OSM)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
OSM, also known as Oncostatin M, is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. However, it is as yet poorly defined and is proving important in liver development, haematopoeisis, inflammation and possibly CNS development. It is also associated with bone formation and destruction. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

Recombinant protein corresponding to aa26-234 of human OSM, fused with His-tag at N-terminus, expressed in E.coli (NP_065391).

AA sequence:
MGSSHHHHHH SSGLVPRGSH MAAIGSCSKE YRVLLGQLQK QTDLMQDTSR LLDPYIRIQG LDVPKLREHC RERPGAFPSE ETLRGLGRRG FLQTLNATLG CVLHRLADLE QRLPKAQDLE RSGLNIEDLE KLQMARPNIL GLRNNIYCMA QLLDNSDTAE PTKAGRGASQ PPTPTPASDA
FQRKLEGCRF LHGYHRFMHS VGRVFSKWGE SPNRSRRHSP HQALRKGVRR

Molecular Weight:
~25.9kD (230aa), confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~25.9kD
Source: E.coli
Purity: ~95% (SDS-PAGE). Purified by conventional chromatography techniques.
Concentration: ~0.5mg/ml (Bradford Assay)
Form: Supplied as a liquid in 20mM Tris-HCl, pH8, 1mM DTT, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Nair B.C., et al. (1992) Science. 255:1430-1432 Miles S.A., et al. (1992) Science. 255:1432-1434.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.