Technical Data
O7030-10
Opioid Receptor, delta, CT, aa360-372
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Technical Data |
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O7030-10 |
Opioid Receptor, delta, CT, aa360-372 |
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Description: The function of the delta-opioid receptors has not been determined. However, recent studies in rodents have linked delta-opioid receptor antagonists with prevention of morphine tolerance and dependence without relinquishing mu-opioid antinociception. In general, opioids modulate numerous central and peripheral processes, including pain perception, neuroendocrine secretion and the immune response. The opioid signal is transduced from receptors through G proteins to various different effectors. Subsequent to G protein activation, several effectors are known to orchestrate the opioid signal. For example, activation of opioid receptors increases phosphatidylinositol turnover, activates K+ channels and reduces adenylyl cyclase and Ca++ channel activities. Applications: Suitable for ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (ABC): 1:800 Stains specific cells in various regions of PLP fixed rat brain sections known to express the delta opioid receptor. Western Blot: 1:800; Band at ~55kD is observed using whole rat brain homogenate. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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