Technical Data
Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor, KOR, KOR-1, OPRK)
Opioid receptors are G-protein coupled receptors. Although the function of k-opioid receptors is unclear, recent evidence indicates that activation of the k-opioid receptor opposes a variety of mu-opioid receptor mediated actions throughout the brain and spinal cord. In general, opioids modulate numerous central and peripheral processes, including pain perception, neuroendocrine secretion and the immune response. The opioid signal is transduced from receptors through G proteins to various different effectors. Subsequent to G protein activation, several effectors are known to orchestrate the opioid signal. For example, activation of opioid receptors increases phosphatidylinositol turnover, activates K+ channels and reduces adenylyl cyclase and Ca++ channel activities.

Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1500 (Whole rat brain homogenate)
Immunocytochemistry: 1:5000 (Stains specific cells in various regions of PLP-fixed rat brain sections known to express the kappa opioid receptor. These include the nucleus accumbens, olfactory tubercule, caudate putamen and portions of the amygdala. Use ABC techniques.)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceRatRabbit
Not Determined
Synthetic peptide corresponding to the C-terminus of rat kappa opioid receptor (carrier protein coupled).
Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Recognizes rat Kappa Opioid Receptor (346-380) and the reactivity of rat Kappa Opioid Receptor is 70%. Does not react to Delta Opioid Receptor (360-372), Delta Opioid Receptor, Mu Opioid Receptor (391-398) and Mu Opioid Receptor. Specific for the COOH terminal.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.