Technical Data
Opioid Receptor 1, mu, aa384-398 (MOR1)
Most actions of exogenous opioids, such as morphine, are mediated through the mu-opioid receptor, including analgesia, tolerance and reward. In general, opioids modulate numerous central and peripheral processes including pain perception, neuroendocrine secretion and the immune response. The opioid signal is transduced from receptors through G proteins to various different effectors. Subsequent to G protein activation, several effectors are known to orchestrate the opioid signal. For example, activation of opioid receptors increases phosphatidyl-inositol turnover, activates K+ channels and reduces adenylyl cyclase and Ca++ channel activities.

Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:6000-1:10,000. Dilute in PBS, 0.3% Triton X-100. Incubate overnight at 4C. Perfusion Fixation: With 500ml 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 20-30 minutes. Post Fixation: 0.1M phosphate buffer, pH 7.4, 4% paraformaldehyde for 1.5 hours at 4C. Section: 10um. Detection: Use biotin/avidin-HRP and Cy3 reagents.
Immunofluorescence (Indirect): 1:500-1:1000 diluted in PBS, 0.3% Triton X-100
Optimal dilutions to be determined by the researcher.

Control Peptide:
O7030-40A: Opioid Receptor 1, mu, aa384-398 Control Peptide (MOR1)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceRatRabbit
Not Determined
Synthetic peptide corresponding to aa384-398, NHQLENLEAETAPLP from the C-terminus of the cloned rat mu opioid receptor-1 (bovine thyroglobulin coupled by gluteraldehyde).
Supplied as a liquid, 0.09% sodium azide.
Recognizes rat mu opioid receptor-1. Specificity was confirmed by Western Blot analysis and immuboisolation studies. Species Crosseactivity: human, monkey and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arvidsson, U., Riedl, M., Chakrabarti, S., Lee, J., Nakano, A., Dado, R., Loh, H., Law, P., Wessendorf, M., Elde, P.: Distribution and targeting of a mu-opioid receptor (MOR1) in brain and spinal cord. J. Neurosci. 15(5): 3328-3341 (1995). 2. J. Comp. Neurol. 371: 522-536 (1996). 3. J. Biol. Chem. 271: 21422-21429 (1996).