Description:
Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:10,000-1:1000. Use 50-100ng of control per well.
Western Blot: 1-10ug/ml (ECL)
Optimal dilution to be determined by the researcher.
Control Peptide:
O7500-10: Orexin A, Mouse, Control Peptide (Hypocretin A).
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Synthetic peptide, 18aa of mouse Orexin A (KLH coupled). Epitope: N-terminus. Species sequence homology: rat, porcine, ovine, bovine, canine, human 100% and chicken 81%. |
| Purity: |
| Purified by antigen affinity chromatography. |
| Form |
| Supplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS. |
| Specificity: |
| Recognizes the full-length (1-33 aa) of mouse Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed pre-pro-orexin (130 aa), the anti-Orexin A antibodies may also react with prepro orexins. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Sakurai, T. et al. (1998) Cell, 92, 573–585. 2. DeLecca L et al (1998) PNAS 95, 322–327. 3. Ciriello J, 2003, Brain Research, 967, 123-131. 4. Ouedraogo R., 2003, Diabetes, Jan 2003; 52: 111- 117. 5. deOliveira CVR, 2003, Am J Physiol Heart Circ Physiol 284: H1369. 6. Satoh S, 2003, Eur. J. Neuroscience 17, 1635. 7. Davis SF, 2003, J. Neurosci., 23: 3844 - 3854. 8. Ciriello J, 2003, Brain Research, 991, 84-95. 9. Ehrstrom M., 2003, Am J Physiol Gastrointest Liver Physiol, 285, 688. 10. Ciriello J, 2003, Am J Physiol Regul. Int. Comp Physiol, 284: 1611. 11. deOliveira CVR, 2003, Brain Research 978, 14-23.
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