Technical Data
O7500-05
Orexin A (Hypocretin A)
Description:
Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:1000. Use 50-100ng of control per well.
Western Blot: 1-10ug/ml (ECL)
Optimal dilution to be determined by the researcher.

Control Peptide:
O7500-10: Orexin A, Mouse, Control Peptide (Hypocretin A).

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide, 18aa of mouse Orexin A (KLH coupled). Epitope: N-terminus. Species sequence homology: rat, porcine, ovine, bovine, canine, human 100% and chicken 81%.
Purity:
Purified by antigen affinity chromatography.
Form
Supplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes the full-length (1-33 aa) of mouse Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed pre-pro-orexin (130 aa), the anti-Orexin A antibodies may also react with prepro orexins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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