Technical Data
Orexin A (Hypocretin A)
Hypothalamic neuropeptides associated with feeding behavior, binding and activating two closely related Orexin receptors—G-protein coupled receptors (GPCRs) OX1R and OX2R.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:1000. Use 50-100ng of control per well.
Western Blot: 1-10ug/ml (ECL)
Optimal dilution to be determined by the researcher.

Control Peptide:
O7500-10: Orexin A, Mouse, Control Peptide (Hypocretin A).

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
PabIgGAffinity Purified
100ug-20°CBlue IceMouseRabbit
Synthetic peptide, 18aa of mouse Orexin A (KLH coupled). Epitope: N-terminus. Species sequence homology: rat, porcine, ovine, bovine, canine, human 100% and chicken 81%.
Purified by antigen affinity chromatography.
Supplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.
Recognizes the full-length (1-33 aa) of mouse Orexin-A. The epitope is not found in Orexin B. Since Orexin A and Orexin B are derived from an unprocessed pre-pro-orexin (130 aa), the anti-Orexin A antibodies may also react with prepro orexins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sakurai, T. et al. (1998) Cell, 92, 573–585. 2. DeLecca L et al (1998) PNAS 95, 322–327. 3. Ciriello J, 2003, Brain Research, 967, 123-131. 4. Ouedraogo R., 2003, Diabetes, Jan 2003; 52: 111- 117. 5. deOliveira CVR, 2003, Am J Physiol Heart Circ Physiol 284: H1369. 6. Satoh S, 2003, Eur. J. Neuroscience 17, 1635. 7. Davis SF, 2003, J. Neurosci., 23: 3844 - 3854. 8. Ciriello J, 2003, Brain Research, 991, 84-95. 9. Ehrstrom M., 2003, Am J Physiol Gastrointest Liver Physiol, 285, 688. 10. Ciriello J, 2003, Am J Physiol Regul. Int. Comp Physiol, 284: 1611. 11. deOliveira CVR, 2003, Brain Research 978, 14-23.