Technical Data
O8056-04
Osteoclast stimulating factor 1, 1-217 aa, Recombinant, Human, aa1-217 (OSTF1, SH3P2, OSF)
100ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Osteoclast-stimulating factor-1 (OSTF1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast (OCL) formation and bone resorption. OSTF1 is highly expressed in OCL and contains structural domains such as SH3 and ankyrin repeats, which are potentially involved in protein-protein interactions. OSTF1 is ubiquitously expressed analogous to other regulators of OCL activity such as c-Src or transforming growth factor . Recombinant human OSTF1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

AA Sequence:
MSKPPPKPVK PGEGGQVKVF RALYTFEPRT PDELYFEEGD IIYITDMSDT NWWKGTSKGR TGLIPSNYVA EQAESIDNPL HEAAKRGNLS
WLRECLDNRV GVNGLDKAGS TALYWACHGG HKDIVEMLFT QPNIELNQQN KLGDTALHAA AWKGYADIVQ LFLAKGARTD LRNIEKKLAF
DMATNAACAS LLKKKQGTDA VRTLSNAEDY LDDEDSDLEH HHHHH

Molecular Weight: 25.1kD (225 aa)

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
25.1kD
Source: E. coli
Purity: 95% by SDS-PAGE
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 0.5mM DTT, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Kurihara N., et al. (2001) J Bio Chem. 276(44):41035-9 2. Reddy S., et al. (1998) Cell Physiol. 177(4):636-45.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.