Technical Data
O8057
Osteoblast Specific Factor 2 (OSF-2, Periostin, Fasciclin-I like)
Description:
Periostin is a disulfide linked 90kD, 811 amino acid protein originally isolated as a osteoblast-specific factor that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading. Additionally, periostin expression has previously been shown to be significantly increased by both transforming growth factor beta-1(TGFbeta1) and bone morphogenetic protein (BMP-2). OSF-2 has a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain and a C-terminal domain. The fourfold repeated domain of OSF-2 shows homology with the insect protein fasciclin. Periostin mRNA is expressed in the developing mouse embryonic and fetal heart, and that it is localized to the endocardial cushions that ultimately divide the primitive heart tube into a four-chambered heart.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Recombinant human OSF-2 consisting of a protein with N-terminal 75kD fusion HisTag, containing 648 aa residues of human OSF-2 and 23 additional aa residues expressed in E. coli.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 50ul of sterile ddH2O.
Specificity:
Recognizes human Osteoblast Specific Factor 2. Species Crossreactivity: rat, chicken, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A. J Bone Miner Res. 1999; 14: 1239-49. 2. Takeshita S, Kikuno R, Tezuka K, Amann E. Biochem J. 1993; 294 (Pt1): 271-8. 3. Ulstrup JC, Jeansson S, Wiker HG, Harboe M. Infect Immun. 1995; 63: 672-5.