		Technical Data
O8060-13	Osteocalcin (Bone-Gla-Protein, BGP)

Description:
Osteocalcin is single-chain (MW 5800) vitamin K-dependent protein produced by osteoblasts and found in high concentrations in bone.

Applications:
Suitable for use in RIA, ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution
ELISA: 100-1000ug/ml. Coat plate with Osteocalcin at 5-10ug/ml.
Western Blot: 10ug/ml
Immunohistochemistry: 10-15ug/ml
Note: Assays require 1-2mM CaCl2.
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	8.H.13	Highly Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Bovine	Mouse

Concentration:

~5-10mg/ml

Immunogen:

Native bovine bone osteocalcin Extinction Coefficient: E 1%1cm, 280nm=14.0

Purity:

Purified by Ion Exchange chromatography.

Form

Supplied as a liquid in 50% glycerol, H2O.

Specificity:

Recognizes human and bovine bone osteocalcin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.