Technical Data
O8060-13
Osteocalcin (Bone-Gla-Protein, BGP)
Description:
Osteocalcin is single-chain (MW 5800) vitamin K-dependent protein produced by osteoblasts and found in high concentrations in bone.

Applications:
Suitable for use in RIA, ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution
ELISA: 100-1000ug/ml. Coat plate with Osteocalcin at 5-10ug/ml.
Western Blot: 10ug/ml
Immunohistochemistry: 10-15ug/ml
Note: Assays require 1-2mM CaCl2.
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18.H.13Highly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceBovineMouse
Concentration:
~5-10mg/ml
Immunogen:
Native bovine bone osteocalcin Extinction Coefficient: E 1%1cm, 280nm=14.0
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in 50% glycerol, H2O.
Specificity:
Recognizes human and bovine bone osteocalcin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.