Technical Data
O8060-14F
Osteocalcin, aa21-31 (Bone-Gla-Protein, BGP)
Description:
Osteocalcin, also known as Bone-Gla-Protein (BGP), is a single chain (Mr=5800) vitamin K dependent protein produced by osteoblasts. It is found in high concentrations in bone (1-4). Post-translational modification by a vitamin K dependent carboxylase produces three g-carboxyglutamic acid residues at positions 17, 21 and 24. The mature protein contains 49 amino acids and a single intrachain disulfide bond joining Cys23 to Cys29. The secondary structure is highly calcium dependent and contains 14% alpha-helix, 20% beta-sheet and 67% random form in the presence of calcium, and 1% alpha-helix, 20% beta-sheet and 79% random form in the absence of calcium (4). BGP binds to phospholipid vesicles in the presence of calcium ions (Kd=6 x 10e-6 M) (5). BGP also binds hydroxyapatite and is an efficient inhibitor of hydroxyapatite seeded crystal growth, suggesting a regulatory role in bone mineralization (6). Although the fundamental role of BGP in vivo remains elusive, in vitro experimentation suggests roles in both bone resorption and bone formation. BGP has been shown to function as a chemo-attractant in an in vitro macrophage mediated bone resorption system. The serum concentration of BGP has been linked to bone formation rates, and BGP levels are currently used in research assessment of osteoporosis and other metabolic bone disease states (7).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen sections; weak on paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG36D628Highly Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceBovineMouse
Concentration:
~2mg/ml
Immunogen:
Bovine osteocalcin
Purity:
Purified by ammonium sulfate precipitation and ion exchange chromatography.
Form
Supplied as a lyophilized powder from TBS, pH 7.5, 1% BSA and 0.1% sodium azide. Reconstitute with 50ul sterile, ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes aa 21-31 of bovine osteocalcin. Species Crossreactivity: human, rat, goat, sheep, porcine and rabbit Does not cross-react with mouse osteocalcin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Characterization of a gamma-carboxyglutamic acid-containing protein from bone: P.A. Price, et al.; PNAS 73, 1447 (1976) 2. Vitamin K-dependent formation of bone Gla protein (osteocalcin) and its function: P.A. Price; Vitam. Horm. 42, 65 (1985) 3. A one step sandwich enzyme immunoassay for gamma-carboxylated osteocalcin using monoclonal antibodies: N. Koyama, et al.; J. Immunol. Methods 139, 17 (1991) 4. Undercarboxylated osteocalcin measured with a specific immunoassay predicts hip fracture in elderly women: the EPIDOS Study: P. Vergnaud, et al.; J. Clin. Endocrinol. Metab. 82, 719 (1997)