Technical Data
Osteocalcin, aa1-22 (Bone-Gla-Protein, BGP)
Suitable for use in RIA and ELISA. Other applications not tested.

Recommended Dilution:
RIA: 1::2000
ELISA: 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined
Synthetic peptide corresponding to amino acids 1-22 of human Osteocalcin (MRALTLLALLALAALCIAGQAG).
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O or 40-50% glycerol.
Recognizes human osteocalcin (1-22) mature form, osteocalcin (1-44) and osteocalcin (1-49). No reactivity with human bone sialoprotein, human osteopontin and human osteonectin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Price, P.A., et al., in Bone and Mineral Research, Vol. 1, ed. Peck, W., pp. 157-190, Ansterdam: Escerpta Medica (1983).2. Hauschka, P.V., et al., PNAS USA 73: 1447 (1975).3. Gundberg, C.M., et al., Methods Enzymol. 107: 516 (1984).4. Delmas, P.D., et al., Biochemistry 23: 4720 (1984).5. Gendreau, M.A., et al., J. Biol. Chem. 264: 6972 (1989).6. Romberg, R.W., et al., Biochemistry 25: 1176 (1986).7. Tracy, R.P., et al., J. Bone Min. Res. 5: 451 (1990). 8. Romberg, R.W., et al., J. Biol. Chem 260: 2728 (1985). 9. Villarreal, X.C., et al., Biochemistry 28: 6483 (1989). 10. Kelm, R.J., et al., Blood 80: 3112 (1992).