Technical Data
O8060-17
Osteocalcin (Bone-Gla-Protein, BGP)
Description:
Osteocalcin is single-chain (MW 5800) vitamin K-dependent protein produced by osteoblasts and found in high concentrations in bone.

This antibody can be used effectively for research of metabolic bone disease and ectopic calcification.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000 (2ug/ml)
Western Blot: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG33G219Highly Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceBovineMouse
Concentration:
~2mg/ml
Immunogen:
Bovine Osteocalcin
Purity:
Purified by ammonium sulfate fractionation and ion exchange chromatography.
Form
Supplied as a lyophilized powder from 20mM TBS, pH 7.5, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile ddH2O.
Specificity:
Recognizes gamma-carboxylated residues 21-31of bovine Osteocalcin (does not react with heat decarboxylated Osteocalcin). Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hauschka, P.U., Lian, J.B., Gallop, P.M., Proc. Natl. Acad. Sci. U.S.A. 72: 3925 (1975).