Technical Data
O8060-17
Osteocalcin (Bone-Gla-Protein, BGP)
Description:
Osteocalcin is single-chain (MW 5800) vitamin K-dependent protein produced by osteoblasts and found in high concentrations in bone.

This antibody can be used effectively for research of metabolic bone disease and ectopic calcification.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000 (2ug/ml)
Western Blot: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
P3U1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG33G219Highly Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceBovineMouse
Concentration:
~2mg/ml
Immunogen:
Bovine Osteocalcin
Purity:
Purified by ammonium sulfate fractionation and ion exchange chromatography.
Form
Supplied as a lyophilized powder from 20mM TBS, pH 7.5, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile ddH2O.
Specificity:
Recognizes gamma-carboxylated residues 21-31of bovine Osteocalcin (does not react with heat decarboxylated Osteocalcin). Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hauschka, P.U., Lian, J.B., Gallop, P.M., Proc. Natl. Acad. Sci. U.S.A. 72: 3925 (1975).