		Technical Data
O8060-18	Osteocalcin (Bone-Gla-Protein, BGP)

Description:
This antibody can be used effectively for research of metabolic bone disease and ectopic calcification (5).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:3000 (0.67ug/ml)
Western Blot: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with bovine Osteocalcin

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	3G220	Highly Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Bovine	Mouse

Concentration:

~2mg/ml

Immunogen:

Bovine Osteocalcin.

Purity:

Purified by ammonium sulfate fractionation and ion exchange chromatography.

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile distilled water or sterile 40-50% glycerol.

Specificity:

Recognizes bovine gamma-carboxylated residues 15-31. Does not react with heat de-carboxylated Osteocalcin. Species Crossreactivity: Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Hauschka, P.U., et al., Proc. Natl. Acad. Sci. U.S.A. 72: 3925 (1975). 2. Towbin, H., et al., Proc. Natl. Acad. Sci. U.S.A. 76: 4350 (1979). 3. Engvall, E. & Perlmann, P., J. Immunol. 109: 129 (1972). 4. Mancini, G., et al., Immunochemistry 2: 235 (1965). 5. Koyama, N., et al., J. Immunological Methods 139: 17 (1991).