Technical Data
O8060-18
Osteocalcin (Bone-Gla-Protein, BGP)
Description:
This antibody can be used effectively for research of metabolic bone disease and ectopic calcification (5).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:3000 (0.67ug/ml)
Western Blot: 2-10ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma cell-line P3U1 with spleen cells of BALB/c mouse after immunization with bovine Osteocalcin

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a3G220Highly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceBovineMouse
Concentration:
~2mg/ml
Immunogen:
Bovine Osteocalcin.
Purity:
Purified by ammonium sulfate fractionation and ion exchange chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 1% BSA, 0.1% sodium azide. Reconstitute with 50ul sterile distilled water or sterile 40-50% glycerol.
Specificity:
Recognizes bovine gamma-carboxylated residues 15-31. Does not react with heat de-carboxylated Osteocalcin. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hauschka, P.U., et al., Proc. Natl. Acad. Sci. U.S.A. 72: 3925 (1975). 2. Towbin, H., et al., Proc. Natl. Acad. Sci. U.S.A. 76: 4350 (1979). 3. Engvall, E. & Perlmann, P., J. Immunol. 109: 129 (1972). 4. Mancini, G., et al., Immunochemistry 2: 235 (1965). 5. Koyama, N., et al., J. Immunological Methods 139: 17 (1991).