Technical Data
O8060-21H
Osteocalcin (Bone-Gla-Protein, BGLAP, BGP, Bone gamma Carboxyglutamate Protein, Gamma Carboxyglutamic Acid-containing Protein, PMF1)
Description:
Osteocalcin (Bone Gla Protein) is a 49aa single chain vitamin K dependent protein (MW 5.8kD), made by osteoblasts that is a major component of the non-collagenous bone matrix. Serum osteocalcin is elevated in diseases characterized by increased bone turnover such as osteoporosis, hyperparathyroidism and Pagets disease, and low in conditions associated with low bone turnover such as hypoparathyroidism and growth hormone deficiency.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:25-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
250ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to human osteocalcin.
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes intact human osteocalcin. Epitope: towards the C-terminal. Species Crossreactivity: canine, bovine, monkey. Does not react with chicken, rat, mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bielby, R. C. et al. (2004) In vitro differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells. Tissue eng. 10(9-10): 1518-1525.
2. Lopes, M. A. et al. (1998) Flow cytometry for assessing biocompatibility. J. Biomed. Mater. Res. 41(4): 649-656.